View source for Repression of heat shock gene expression (ROSE) element

{{Infobox rfam
| Name = Repression of heat shock gene expression (ROSE) element
| image = RF00435.jpg
| width = 
| caption = Predicted [[secondary structure]] and [[sequence conservation]] of ROSE
| Symbol = ROSE
| AltSymbols = 
| Rfam = RF00435
| miRBase = 
| miRBase_family = 
| RNA_type = [[Cis-regulatory element|Cis-reg]]; thermoregulator
| Tax_domain = [[Bacteria]]
| GO = 
| SO = {{SO|0000204}} 
| CAS_number = 
| EntrezGene = 
| HGNCid = 
| OMIM = 
| PDB = 
| RefSeq = 
| Chromosome = 
| Arm = 
| Band = 
| LocusSupplementaryData = 
}}

[[File:PDB 2gio EBI.png|thumb|A representation of the 3D structure of the microROSE RNA. This derived from a solution [[NMR]] structure of the microROSE element.<ref name="pmid16710302">{{cite journal |doi=10.1038/sj.emboj.7601128 |vauthors=Chowdhury S, Maris C, Allain FH, Narberhaus F |title=Molecular basis for temperature sensing by an RNA thermometer |journal=EMBO J |volume=25 |issue=11 |pages=2487–2497 |year=2006 |pmid=16710302 |pmc=1478195}}</ref>]]

The '''repression of heat shock gene expression (ROSE) element''' is an [[cis-regulatory element|RNA element]] found in the [[5' UTR]] of some heat shock protein's [[mRNA]]s. The ROSE element is an [[RNA thermometer]] that negatively regulates [[heat shock]] [[gene expression]]. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the [[Shine-Dalgarno sequence|ribosome binding site]] at normal temperatures. During heat shock however, the structure changes freeing the [[ribosome]] binding site and allowing expression to occur.<ref>{{cite journal | last = Nocker | first = A |author2=Hausherr T |author3=Balsiger S |author4=Krstulovic NP |author5=Hennecke H |author6=Narberhaus F  | year = 2001 | title = A mRNA-based thermosensor controls expression of rhizobial heat shock genes | journal = Nucleic Acids Res | volume = 29 | pages = 4800–4807 | pmid = 11726689 | doi = 10.1093/nar/29.23.4800 | issue = 23 | pmc = 96696}}</ref><ref>{{cite journal | last = Balsiger | first = S |author2=Ragaz C |author3=Baron C |author4=Narberhaus F  | year = 2004 | title = Replicon-Specific Regulation of Small Heat Shock Genes in Agrobacterium tumefaciens | journal = J Bacteriol | volume = 186 | pages = 6824–6829 | pmid = 15466035 | doi = 10.1128/JB.186.20.6824-6829.2004 | issue = 20 | pmc = 522190}}</ref>

A partial structure of this RNA element has been determined using [[NMR]].<ref name="pmid16710302"/>

==ROSE<sub>1</sub> and ROSE<sub>AT2</sub>==
ROSE<sub>1</sub> and ROSE<sub>AT2</sub> are specific examples of ROSE elements.<ref name="Narb06">{{cite journal |vauthors=Narberhaus F, Waldminghaus T, Chowdhury S |title=RNA thermometers |journal=FEMS Microbiol. Rev. |volume=30 |issue=1 |pages=3–16 |date=January 2006 |pmid=16438677 |doi=10.1111/j.1574-6976.2005.004.x |doi-access= }}</ref> ROSE<sub>1</sub> is found in ''[[Bradyrhizobium japonicum]]'' whereas ROSE<sub>AT2</sub> is a closely related element from ''[[Agrobacterium tumefaciens]]''. The two RNA elements have similar [[secondary structure]]s with ROSE<sub>1</sub> having an extra [[stem-loop|hairpin]].<ref name="Narb06" /> All ROSE elements contain a characteristic 'bulged G' opposite the [[Shine-Dalgarno sequence]] binding site, without this [[nucleotide]] the RNA thermometer loses its temperature-sensitivity.<ref>{{cite journal |vauthors=Nocker A, Hausherr T, Balsiger S, Krstulovic NP, Hennecke H, Narberhaus F |title=A mRNA-based thermosensor controls expression of rhizobial heat shock genes |journal=Nucleic Acids Res. |volume=29 |issue=23 |pages=4800–4807 |date=December 2001 |pmid=11726689 |pmc=96696 |doi= 10.1093/nar/29.23.4800|url=}}</ref>

==IpbA==
Expression of the heat shock protein IpbA in species of ''[[Pseudomonas]]'' is controlled by a ROSE-type RNA thermometer. This thermometer only consists of two hairpins. It inhibits [[Translation (genetics)|translation]] of IpbA at low temperatures and permits translation when temperature increases.<ref>{{cite journal|last=Krajewski|first=SS|author2=Nagel, M |author3=Narberhaus, F |title=Short ROSE-Like RNA Thermometers Control IbpA Synthesis in Pseudomonas Species.|journal=PLOS ONE|date=2013|volume=8|issue=5|pages=e65168|pmid=23741480|doi=10.1371/journal.pone.0065168|pmc=3669281|bibcode=2013PLoSO...865168K|doi-access=free}}</ref>

== ''rhlA'' and ''lsdI'' ROSE-like elements ==
''[[Pseudomonas aeruginosa]]'' [[quorum sensing]]-dependent virulence factors can be thermoregulated. ROSE thermometer present in the 5'UTR of ''rhlA'' and ROSE-like thermometer in the 5'UTR of ''lasI'' block the translation at lower temperatures.<ref>{{Cite journal|last1=Grosso-Becerra|first1=María Victoria|last2=Croda-García|first2=Gerardo|last3=Merino|first3=Enrique|last4=Servín-González|first4=Luis|last5=Mojica-Espinosa|first5=Raúl|last6=Soberón-Chávez|first6=Gloria|date=2014-10-28|title=Regulation of Pseudomonas aeruginosa virulence factors by two novel RNA thermometers|journal=Proceedings of the National Academy of Sciences of the United States of America|volume=111|issue=43|pages=15562–15567|doi=10.1073/pnas.1402536111|issn=1091-6490|pmc=4217398|pmid=25313031|bibcode=2014PNAS..11115562G|doi-access=free}}</ref>

==See also==
* [[Cyanobacterial RNA thermometers|Cyanobacterial RNA thermometer]]
* [[FourU thermometer]]
* [[Hsp90 cis-regulatory element]]

==References==
{{reflist}}

==Further reading==
*{{Cite journal
| last1 = De La Fuente | first1 = M. 
| last2 = Valera | first2 = S. 
| last3 = Martínez-Guitarte | first3 = J. L. 
| doi = 10.1016/j.febslet.2012.10.018 
| title = NcRNAs and thermoregulation: A view in prokaryotes and eukaryotes 
| journal = FEBS Letters 
| volume = 586 
| issue = 23 
| pages = 4061–4069 
| year = 2012 
| pmid = 23098758 
| pmc = 
| doi-access = free 
}}

==External links==
* {{Rfam|id=RF00435|name=Repression of heat shock gene expression (ROSE) element}}

{{DEFAULTSORT:Repression Of Heat Shock Gene Expression (Rose) Element}}
[[Category:Cis-regulatory RNA elements]]
{{molecular-cell-biology-stub}}